Saturation mutagenesis of an Escherichia coli rRNA promoter and initial characterization of promoter variants.

Using oligonucleotide synthesis techniques, we generated Escherichia coli rrnB P1 (rrnB1p according to the nomenclature of B. J. Bachmann and K. B. Low [Microbiol. Rev. 44:1-56, 1980]) promoter fragments containing single base substitutions, insertions, deletions, and multiple mutations, covering th...

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Autors principals: Gaal, T, Barkei, J, Dickson, R R, deBoer, H A, deHaseth, P L, Alavi, H, Gourse, R L
Format: Artigo
Idioma:Inglês
Publicat: 1989
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC210289/
https://ncbi.nlm.nih.gov/pubmed/2527844
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