Saturation mutagenesis of an Escherichia coli rRNA promoter and initial characterization of promoter variants.

Using oligonucleotide synthesis techniques, we generated Escherichia coli rrnB P1 (rrnB1p according to the nomenclature of B. J. Bachmann and K. B. Low [Microbiol. Rev. 44:1-56, 1980]) promoter fragments containing single base substitutions, insertions, deletions, and multiple mutations, covering th...

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Bibliografische gegevens

Gepubliceerd in:	J Bacteriol
Hoofdauteurs:	Gaal, T, Barkei, J, Dickson, R R, deBoer, H A, deHaseth, P L, Alavi, H, Gourse, R L
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 1989
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210289/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2527844/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.9.4852-4861.1989
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