Saturation mutagenesis of an Escherichia coli rRNA promoter and initial characterization of promoter variants.

Using oligonucleotide synthesis techniques, we generated Escherichia coli rrnB P1 (rrnB1p according to the nomenclature of B. J. Bachmann and K. B. Low [Microbiol. Rev. 44:1-56, 1980]) promoter fragments containing single base substitutions, insertions, deletions, and multiple mutations, covering th...

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Bibliografski detalji
Glavni autori: Gaal, T, Barkei, J, Dickson, R R, deBoer, H A, deHaseth, P L, Alavi, H, Gourse, R L
Format: Artigo
Jezik:Inglês
Izdano: 1989
Teme:
Research Article
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC210289/
https://ncbi.nlm.nih.gov/pubmed/2527844
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