Saturation mutagenesis of an Escherichia coli rRNA promoter and initial characterization of promoter variants.

Using oligonucleotide synthesis techniques, we generated Escherichia coli rrnB P1 (rrnB1p according to the nomenclature of B. J. Bachmann and K. B. Low [Microbiol. Rev. 44:1-56, 1980]) promoter fragments containing single base substitutions, insertions, deletions, and multiple mutations, covering th...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Gaal, T, Barkei, J, Dickson, R R, deBoer, H A, deHaseth, P L, Alavi, H, Gourse, R L
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1989
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210289/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2527844/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.9.4852-4861.1989
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