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Engineering subunit association of multisubunit proteins: A dimeric streptavidin
A dimeric streptavidin has been designed by molecular modeling using effective binding free energy calculations that decompose the binding free energy into electrostatic, desolvation, and side chain entropy loss terms. A histidine-127 → aspartic acid (H127D) mutation was sufficient to introduce elec...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
The National Academy of Sciences of the USA
1997
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC21018/ https://ncbi.nlm.nih.gov/pubmed/9177186 |
| Oznake: |
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