Validation of RNA-based molecular clonotype analysis for virus-specific CD8+ T-cells in formaldehyde-fixed specimens isolated from peripheral blood

Recent advances in the field of molecular clonotype analysis have enabled detailed repertoire characterization of viably isolated antigen-specific T cell populations directly ex vivo. However, in the absence of a biologically contained FACS facility, peripheral blood mononuclear cell (PBMC) preparat...

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Main Authors: van Bockel, David, Price, David A., Asher, Tedi E., Venturi, Vanessa, Suzuki, Kazuo, Warton, Kristina, Davenport, Miles P., Cooper, David A., Douek, Daniel C., Kelleher, Anthony D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2080792/
https://ncbi.nlm.nih.gov/pubmed/17716684
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jim.2007.07.016
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