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Genetic Method To Analyze Essential Genes of Escherichia coli

The genetic analysis of essential genes has been generally restricted to the use of conditional mutations, or inactivating chromosomal mutations, which require a complementing plasmid that must either be counterselected or lost to measure a phenotype. These approaches are limited because they do not...

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Detalhes bibliográficos
Main Authors: Hupert-Kocurek, Katarzyna, Sage, Jay M., Makowska-Grzyska, Magdalena, Kaguni, Jon M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2074942/
https://ncbi.nlm.nih.gov/pubmed/17873068
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01756-07
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