Cloning, sequencing, and expression of thermophilic Bacillus sp. strain TB-90 urease gene complex in Escherichia coli.

The urease of thermophilic Bacillus sp. strain TB-90 is composed of three subunits with molecular masses of 61, 12, and 11 kDa. By using synthetic oligonucleotide probes based on N-terminal amino acid sequences of each subunit, we cloned a 3.2-kb EcoRI fragment of TB-90 genomic DNA. Moreover, we clo...

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Dettagli Bibliografici

Pubblicato in:	J Bacteriol
Autori principali:	Maeda, M, Hidaka, M, Nakamura, A, Masaki, H, Uozumi, T
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1994
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205067/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8288539/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.2.432-442.1994
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