Cloning, sequencing, and expression of thermophilic Bacillus sp. strain TB-90 urease gene complex in Escherichia coli.

The urease of thermophilic Bacillus sp. strain TB-90 is composed of three subunits with molecular masses of 61, 12, and 11 kDa. By using synthetic oligonucleotide probes based on N-terminal amino acid sequences of each subunit, we cloned a 3.2-kb EcoRI fragment of TB-90 genomic DNA. Moreover, we clo...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Maeda, M, Hidaka, M, Nakamura, A, Masaki, H, Uozumi, T
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1994
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205067/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8288539/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.2.432-442.1994
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