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Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants.

Clones carrying the gene encoding a proteinase were isolated from Clarke and Carbon's collection, using a chromogenic substrate, N-benzyloxycarbonyl-L-phenylalanine beta-naphthyl ester. The three clones isolated, pLC6-33, pLC13-1, and pLC36-46, shared the same chromosomal DNA region. A 0.9-kb S...

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Bibliografiska uppgifter
Huvudupphovsmän: Ichihara, S, Matsubara, Y, Kato, C, Akasaka, K, Mizushima, S
Materialtyp: Artigo
Språk:Inglês
Publicerad: 1993
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC193016/
https://ncbi.nlm.nih.gov/pubmed/8432696
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