Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants.

Clones carrying the gene encoding a proteinase were isolated from Clarke and Carbon's collection, using a chromogenic substrate, N-benzyloxycarbonyl-L-phenylalanine beta-naphthyl ester. The three clones isolated, pLC6-33, pLC13-1, and pLC36-46, shared the same chromosomal DNA region. A 0.9-kb Sau3AI...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Ichihara, S, Matsubara, Y, Kato, C, Akasaka, K, Mizushima, S
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1993
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC193016/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8432696/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.175.4.1032-1037.1993
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