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Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a beta-mannanase from the extremely thermophilic bacterium "Caldocellum saccharolyticum".

A lambda recombinant phage expressing beta-mannanase activity in Escherichia coli has been isolated from a genomic library of the extremely thermophilic anaerobe "Caldocellum saccharolyticum." The gene was cloned into pBR322 on a 5-kb BamHI fragment, and its location was obtained by deletion analysi...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Principais autores: Lüthi, E, Jasmat, N B, Grayling, R A, Love, D R, Bergquist, P L
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC182781/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2039230/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.57.3.694-700.1991
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