Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cells.
The 3-isopropylmalate dehydrogenase gene from an extreme thermophile, Thermus thermophilus, was not expressed in Escherichia coli unless a palindromic structure around the ribosome binding site was eliminated or a leader open reading frame was introduced into the upstream flanking region of the gene...
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| Publicado no: | J Bacteriol |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
1994
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205423/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8169232/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.9.2767-2770.1994 |
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