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Overexpression of genes of an extreme thermophile Thermus thermophilus, in Escherichia coli cells.

The 3-isopropylmalate dehydrogenase gene from an extreme thermophile, Thermus thermophilus, was not expressed in Escherichia coli unless a palindromic structure around the ribosome binding site was eliminated or a leader open reading frame was introduced into the upstream flanking region of the gene...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Ishida, M, Oshima, T
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1994
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC205423/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8169232/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.176.9.2767-2770.1994
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