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Formation, regeneration, and transfection of Lactobacillus plantarum protoplasts.

Protoplasts of L. plantarum were produced by mutanolysin-lysozyme digestion at 50 degrees C and regenerated at a frequency of 1.6 to 3.8%. The addition of Tween 80 to the growth medium increased the length of time required for protoplast formation. When transfected with bacteriophage B2 DNA, transfe...

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Dettagli Bibliografici
Autori principali: Cosby, W M, Casas, I A, Dobrogosz, W J
Natura: Artigo
Lingua:Inglês
Pubblicazione: 1988
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC204341/
https://ncbi.nlm.nih.gov/pubmed/3214148
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