Formation, regeneration, and transfection of Lactobacillus plantarum protoplasts.

Protoplasts of L. plantarum were produced by mutanolysin-lysozyme digestion at 50 degrees C and regenerated at a frequency of 1.6 to 3.8%. The addition of Tween 80 to the growth medium increased the length of time required for protoplast formation. When transfected with bacteriophage B2 DNA, transfe...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Cosby, W M, Casas, I A, Dobrogosz, W J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1988
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204341/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3214148/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.11.2599-2602.1988
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