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Formation, regeneration, and transfection of Lactobacillus plantarum protoplasts.
Protoplasts of L. plantarum were produced by mutanolysin-lysozyme digestion at 50 degrees C and regenerated at a frequency of 1.6 to 3.8%. The addition of Tween 80 to the growth medium increased the length of time required for protoplast formation. When transfected with bacteriophage B2 DNA, transfe...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
1988
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC204341/ https://ncbi.nlm.nih.gov/pubmed/3214148 |
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