Formation, regeneration, and transfection of Lactobacillus plantarum protoplasts.
Protoplasts of L. plantarum were produced by mutanolysin-lysozyme digestion at 50 degrees C and regenerated at a frequency of 1.6 to 3.8%. The addition of Tween 80 to the growth medium increased the length of time required for protoplast formation. When transfected with bacteriophage B2 DNA, transfe...
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| Udgivet i: | Appl Environ Microbiol |
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| Principais autores: | , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Microbiology (ASM)
1988
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204341/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3214148/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.54.11.2599-2602.1988 |
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