Preparation and regeneration of bacteriophage PL-1 enzyme-induced Lactobacillus casei protoplasts.

Lactobacillus casei ATCC 27092 protoplasts were obtained by treatment with a bacteriolytic enzyme, which was produced in the PL-1 phage lysates of this bacterium, at 37 degrees C for 1.5 h in 50 mM Tris hydrochloride buffer (pH 7.2) containing 20% sucrose and 10 mM MgSO4. The protoplasts lacked the...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Watanabe, K, Hayashida, M, Nakashima, Y, Hayashi, S
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 1987
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC204176/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3122657/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.11.2686-2688.1987
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