Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus.

The conditions for optimal formation and regeneration of protoplasts of Streptomyces clavuligerus were established. The optimal temperature for regeneration of protoplasts and for transformation was 26 degrees C in three different regeneration media. The best efficiency of transformation was obtaine...

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Bibliografski detalji

Izdano u:	Appl Environ Microbiol
Glavni autori:	Garcia-Dominguez, M, Martin, J F, Mahro, B, Demain, A L, Liras, P
Format:	Artigo
Jezik:	Inglês
Izdano:	American Society for Microbiology (ASM) 1987
Teme:	Research Article
Online pristup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203873/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3606113/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.6.1376-1381.1987
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