Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus.

The conditions for optimal formation and regeneration of protoplasts of Streptomyces clavuligerus were established. The optimal temperature for regeneration of protoplasts and for transformation was 26 degrees C in three different regeneration media. The best efficiency of transformation was obtaine...

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Библиографические подробности

Опубликовано в::	Appl Environ Microbiol
Главные авторы:	Garcia-Dominguez, M, Martin, J F, Mahro, B, Demain, A L, Liras, P
Формат:	Artigo
Язык:	Inglês
Опубликовано:	American Society for Microbiology (ASM) 1987
Предметы:	Research Article
Online-ссылка:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203873/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3606113/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.6.1376-1381.1987
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