Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus.

The conditions for optimal formation and regeneration of protoplasts of Streptomyces clavuligerus were established. The optimal temperature for regeneration of protoplasts and for transformation was 26 degrees C in three different regeneration media. The best efficiency of transformation was obtaine...

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Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Garcia-Dominguez, M, Martin, J F, Mahro, B, Demain, A L, Liras, P
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1987
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203873/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3606113/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.6.1376-1381.1987
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