Efficient plasmid transformation of the beta-lactam producer Streptomyces clavuligerus.

The conditions for optimal formation and regeneration of protoplasts of Streptomyces clavuligerus were established. The optimal temperature for regeneration of protoplasts and for transformation was 26 degrees C in three different regeneration media. The best efficiency of transformation was obtaine...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Garcia-Dominguez, M, Martin, J F, Mahro, B, Demain, A L, Liras, P
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1987
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203873/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3606113/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.53.6.1376-1381.1987
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