Ligation mediated PCR performed at low denaturation temperatures—PCR melting profiles

We show that using low denaturation temperatures (80–88°C) during ligation mediated PCR (LM PCR) of bacterial DNA leads to the amplification of limited sets of the less stable DNA fragments. A set of electrophoretic patterns of such fragments obtained at different denaturation temperatures forms the...

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Pubblicato in:	Nucleic Acids Res
Autori principali:	Masny, Aleksander, Płucienniczak, Andrzej
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	Oxford University Press 2003
Soggetti:	NAR Methods Online
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203340/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12954790/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gng116
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