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Ligation mediated PCR performed at low denaturation temperatures—PCR melting profiles
We show that using low denaturation temperatures (80–88°C) during ligation mediated PCR (LM PCR) of bacterial DNA leads to the amplification of limited sets of the less stable DNA fragments. A set of electrophoretic patterns of such fragments obtained at different denaturation temperatures forms the...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC203340/ https://ncbi.nlm.nih.gov/pubmed/12954790 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng116 |
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