Automated one-step DNA sequencing based on nanoliter reaction volumes and capillary electrophoresis
An integrated system with a nano-reactor for cycle-sequencing reaction coupled to on-line purification and capillary gel electrophoresis has been demonstrated. Fifty nanoliters of reagent solution, which includes dye-labeled terminators, polymerase, BSA and template, was aspirated and mixed with the...
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| Pubblicato in: | Nucleic Acids Res |
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| Autori principali: | , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Oxford University Press
2000
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC102692/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10908366/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/28.15.e73 |
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