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Ligation mediated PCR performed at low denaturation temperatures—PCR melting profiles

We show that using low denaturation temperatures (80–88°C) during ligation mediated PCR (LM PCR) of bacterial DNA leads to the amplification of limited sets of the less stable DNA fragments. A set of electrophoretic patterns of such fragments obtained at different denaturation temperatures forms the...

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Bibliografiske detaljer
Main Authors: Masny, Aleksander, Płucienniczak, Andrzej
Format: Artigo
Sprog:Inglês
Udgivet: Oxford University Press 2003
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC203340/
https://ncbi.nlm.nih.gov/pubmed/12954790
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gng116
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