High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media
An efficient method for genetic transformation of lactococci by electroporation is presented. Highly competent lactococci for electrotransformation were obtained by growing cells in media containing high concentrations of glycine and 0.5 M sucrose as the osmotic stabilizers. These cells could be sto...
محفوظ في:
| الحاوية / القاعدة: | Appl Environ Microbiol |
|---|---|
| المؤلفون الرئيسيون: | , |
| التنسيق: | Artigo |
| اللغة: | Inglês |
| منشور في: |
American Society for Microbiology (ASM)
1989
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| الموضوعات: | |
| الوصول للمادة أونلاين: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203233/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348073/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.12.3119-3123.1989 |
| الوسوم: |
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