High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media

An efficient method for genetic transformation of lactococci by electroporation is presented. Highly competent lactococci for electrotransformation were obtained by growing cells in media containing high concentrations of glycine and 0.5 M sucrose as the osmotic stabilizers. These cells could be sto...

Täydet tiedot

Bibliografiset tiedot

Julkaisussa:	Appl Environ Microbiol
Päätekijät:	Holo, Helge, Nes, Ingolf F.
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	American Society for Microbiology (ASM) 1989
Aiheet:	Methods
Linkit:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203233/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348073/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.12.3119-3123.1989
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