High-Frequency Transformation, by Electroporation, of Lactococcus lactis subsp. cremoris Grown with Glycine in Osmotically Stabilized Media

An efficient method for genetic transformation of lactococci by electroporation is presented. Highly competent lactococci for electrotransformation were obtained by growing cells in media containing high concentrations of glycine and 0.5 M sucrose as the osmotic stabilizers. These cells could be sto...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Holo, Helge, Nes, Ingolf F.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1989
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC203233/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16348073/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.12.3119-3123.1989
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