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Genetic transformation of intact Lactococcus lactis subsp. lactis by high-voltage electroporation.

To apply recombinant DNA techniques for genetic manipulation of the industrially important lactococci, an efficient and reliable high-frequency transformation system must be available. High-voltage electric pulses have been demonstrated to enhance uptake of DNA into protoplasts and intact cells of n...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: McIntyre, D A, Harlander, S K
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1989
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC184167/
https://ncbi.nlm.nih.gov/pubmed/2494937
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