Genetic transformation of intact Lactococcus lactis subsp. lactis by high-voltage electroporation.
To apply recombinant DNA techniques for genetic manipulation of the industrially important lactococci, an efficient and reliable high-frequency transformation system must be available. High-voltage electric pulses have been demonstrated to enhance uptake of DNA into protoplasts and intact cells of n...
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| Опубликовано в:: | Appl Environ Microbiol |
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| Главные авторы: | , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
1989
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC184167/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2494937/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.55.3.604-610.1989 |
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