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T4 DNA ligase is more than an effective trap of cyclized dsDNA

T4 DNA ligase is used in standard cyclization assays to trap double-stranded DNA (dsDNA) in low-probability, cyclic or highly bent conformations. The cyclization probability, deduced from the relative yield of cyclized product, can be used in conjunction with statistical mechanical models to extract...

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Autors principals: Yuan, Chongli, Lou, Xiong Wen, Rhoades, Elizabeth, Chen, Huimin, Archer, Lynden A.
Format: Artigo
Idioma:Inglês
Publicat: Oxford University Press 2007
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2018621/
https://ncbi.nlm.nih.gov/pubmed/17686784
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm582
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