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T4 DNA ligase is more than an effective trap of cyclized dsDNA
T4 DNA ligase is used in standard cyclization assays to trap double-stranded DNA (dsDNA) in low-probability, cyclic or highly bent conformations. The cyclization probability, deduced from the relative yield of cyclized product, can be used in conjunction with statistical mechanical models to extract...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Oxford University Press
2007
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2018621/ https://ncbi.nlm.nih.gov/pubmed/17686784 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkm582 |
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