Natural and Electroporation-Mediated Transformation of Methanococcus voltae Protoplasts

The lack of high-efficiency transformation systems has severely impeded genetic research on methanogenic members of the kingdom Archaeobacteria. By using protoplasts of Methanococcus voltae and an integration vector, Mip1, previously shown to impart puromycin resistance, we obtained natural transfor...

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Bibliografiske detaljer
Main Authors: Patel, Girishchandra B., Nash, John H. E., Agnew, Brian J., Sprott, G. Dennis
Format: Artigo
Sprog:Inglês
Udgivet: 1994
Fag:
Genetics and Molecular Biology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC201408/
https://ncbi.nlm.nih.gov/pubmed/16349218
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