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Natural and Electroporation-Mediated Transformation of Methanococcus voltae Protoplasts

The lack of high-efficiency transformation systems has severely impeded genetic research on methanogenic members of the kingdom Archaeobacteria. By using protoplasts of Methanococcus voltae and an integration vector, Mip1, previously shown to impart puromycin resistance, we obtained natural transfor...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Patel, Girishchandra B., Nash, John H. E., Agnew, Brian J., Sprott, G. Dennis
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1994
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC201408/
https://ncbi.nlm.nih.gov/pubmed/16349218
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