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Natural and Electroporation-Mediated Transformation of Methanococcus voltae Protoplasts

The lack of high-efficiency transformation systems has severely impeded genetic research on methanogenic members of the kingdom Archaeobacteria. By using protoplasts of Methanococcus voltae and an integration vector, Mip1, previously shown to impart puromycin resistance, we obtained natural transfor...

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Détails bibliographiques
Auteurs principaux: Patel, Girishchandra B., Nash, John H. E., Agnew, Brian J., Sprott, G. Dennis
Format: Artigo
Langue:Inglês
Publié: 1994
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC201408/
https://ncbi.nlm.nih.gov/pubmed/16349218
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