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Natural and Electroporation-Mediated Transformation of Methanococcus voltae Protoplasts

The lack of high-efficiency transformation systems has severely impeded genetic research on methanogenic members of the kingdom Archaeobacteria. By using protoplasts of Methanococcus voltae and an integration vector, Mip1, previously shown to impart puromycin resistance, we obtained natural transfor...

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Detalles Bibliográficos
Publicado en:Appl Environ Microbiol
Principais autores: Patel, Girishchandra B., Nash, John H. E., Agnew, Brian J., Sprott, G. Dennis
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1994
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC201408/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16349218/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.60.3.903-907.1994
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