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Natural and Electroporation-Mediated Transformation of Methanococcus voltae Protoplasts

The lack of high-efficiency transformation systems has severely impeded genetic research on methanogenic members of the kingdom Archaeobacteria. By using protoplasts of Methanococcus voltae and an integration vector, Mip1, previously shown to impart puromycin resistance, we obtained natural transfor...

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Detalhes bibliográficos
Main Authors: Patel, Girishchandra B., Nash, John H. E., Agnew, Brian J., Sprott, G. Dennis
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC201408/
https://ncbi.nlm.nih.gov/pubmed/16349218
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