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Role of the catalytic metal during polymerization by DNA polymerase lambda

The incorporation of dNMPs into DNA by polymerases involves a phosphoryl transfer reaction hypothesized to require two divalent metal ions. Here we investigate this hypothesis using as a model human DNA polymerase λ (Pol λ), an enzyme suggested to be activated in vivo by manganese. We report the cry...

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Autori principali: Garcia-Diaz, Miguel, Bebenek, Katarzyna, Krahn, Joseph M., Pedersen, Lars C., Kunkel, Thomas A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2007
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1989765/
https://ncbi.nlm.nih.gov/pubmed/17475573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.dnarep.2007.03.005
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