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A 1H n.m.r. study of the role of the glutamate moiety in the binding of methotrexate to Lactobacillus casei dihydrofolate reductase.

The binding of a series of amide derivatives of methotrexate to Lactobacillus casei dihydrofolate reductase has been studied by inhibition constant measurements and by 1H n.m.r. spectroscopy. Amide modification of the alpha-carboxylate of methotrexate was found to prevent interaction of the gamma-ca...

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Autors principals: Antonjuk, D. J., Birdsall, B., Cheung, H. T., Clore, G. M., Feeney, J., Gronenborn, A., Roberts, G. C., Tran, T. Q.
Format: Artigo
Idioma:Inglês
Publicat: Nature Publishing Group 1984
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1986890/
https://ncbi.nlm.nih.gov/pubmed/6423020
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