Large-scale purification and characterization of dihydrofolate reductase from a methotrexate-resistant strain of Lactobacillus casei.

Dihydrofolate reductase has been purified from a methotrexate-resistant strain of Lactobacillus casei NCB 6375. By careful attention to growth conditions, up to 2.5 g of enzyme is obtained from a 400 litre culture. The purification procedure, involving poly-ethyleneimine treatment, DEAE-cellulose ch...

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Detalhes bibliográficos
Main Authors: Dann, J G, Ostler, G, Bjur, R A, King, R W, Scudder, P, Turner, P C, Roberts, G C, Burgen, A S
Formato: Artigo
Idioma:Inglês
Publicado em: 1976
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1163897/
https://ncbi.nlm.nih.gov/pubmed/10886
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