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Effect of target secondary structure on RNAi efficiency

RNA interference (RNAi) mediated by small interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) has become a powerful tool for gene knockdown studies. However, the levels of knockdown vary greatly. Here, we examine the effect of target disruption energy, a novel measure of target accessibility, a...

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Detalhes bibliográficos
Main Authors: Shao, Yu, Chan, Chi Yu, Maliyekkel, Anil, Lawrence, Charles E., Roninson, Igor B., Ding, Ye
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1986803/
https://ncbi.nlm.nih.gov/pubmed/17684233
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.546207
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