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Transcriptional sequencing: A method for DNA sequencing using RNA polymerase

We have developed a sequencing method based on the RNA polymerase chain termination reaction with rhodamine dye attached to 3′-deoxynucleoside triphosphate (3′-dNTP). This method enables us to conduct a rapid isothermal sequencing reaction in <30 min, to reduce the amount of template required, and t...

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Guardat en:
Publicat a:Proc Natl Acad Sci U S A
Autors principals: Sasaki, Nobuya, Izawa, Masaki, Watahiki, Masanori, Ozawa, Kaori, Tanaka, Takumi, Yoneda, Yuko, Matsuura, Shuji, Carninci, Piero, Muramatsu, Masami, Okazaki, Yasushi, Hayashizaki, Yoshihide
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 1998
Matèries:
Biological Sciences
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC19857/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9520387/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.7.3455
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