Transcriptional sequencing: A method for DNA sequencing using RNA polymerase
We have developed a sequencing method based on the RNA polymerase chain termination reaction with rhodamine dye attached to 3′-deoxynucleoside triphosphate (3′-dNTP). This method enables us to conduct a rapid isothermal sequencing reaction in <30 min, to reduce the amount of template required, and t...
Uloženo v:
| Vydáno v: | Proc Natl Acad Sci U S A |
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| Hlavní autoři: | , , , , , , , , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
National Academy of Sciences
1998
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC19857/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9520387/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.7.3455 |
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