Transcriptional sequencing: A method for DNA sequencing using RNA polymerase

We have developed a sequencing method based on the RNA polymerase chain termination reaction with rhodamine dye attached to 3′-deoxynucleoside triphosphate (3′-dNTP). This method enables us to conduct a rapid isothermal sequencing reaction in <30 min, to reduce the amount of template required, an...

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Detalhes bibliográficos
Main Authors: Sasaki, Nobuya, Izawa, Masaki, Watahiki, Masanori, Ozawa, Kaori, Tanaka, Takumi, Yoneda, Yuko, Matsuura, Shuji, Carninci, Piero, Muramatsu, Masami, Okazaki, Yasushi, Hayashizaki, Yoshihide
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 1998
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC19857/
https://ncbi.nlm.nih.gov/pubmed/9520387
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