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Purification, crystallization and preliminary X-ray analysis of a hexameric β-glucosidase from wheat
The wheat β-glucosidase TaGlu1b, which is only active in a hexameric form, was tagged with 6×His at the N-terminus, overexpressed in Escherichia coli and purified in two steps. The protein complexed with a substrate aglycone was crystallized at 293 K from a solution containing 10 mM HEPES pH 7.2, 1 ...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
International Union of Crystallography
2005
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1978116/ https://ncbi.nlm.nih.gov/pubmed/16511181 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105027028 |
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