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Expression, purification, crystallization and preliminary crystallographic analysis of a deblocking aminopeptidase from Pyrococcus horikoshii

The deblocking aminopeptidase (DAP) of Pyrococcus horikoshii is a hyperthermophilic exoprotease that cleaves the N-terminal amino acid of peptide substrates with a putative deblocking activity for acylated peptides. DAP has been found to be homologous to a tetrahedral aminopeptidase from the halophi...

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Detalhes bibliográficos
Main Authors: Porciero, Sophie, Receveur-Bréchot, Véronique, Mori, Kazushige, Franzetti, Bruno, Roussel, Alain
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2005
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1952244/
https://ncbi.nlm.nih.gov/pubmed/16511005
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309105001910
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