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Self-Formed Adaptor PCR: a Simple and Efficient Method for Chromosome Walking
We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequen...
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| Главные авторы: | , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology
2007
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1951051/ https://ncbi.nlm.nih.gov/pubmed/17483260 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02973-06 |
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