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Simple Absolute Quantification Method Correcting for Quantitative PCR Efficiency Variations for Microbial Community Samples

Real-time quantitative PCR (qPCR) is a widely used technique in microbial community analysis, allowing the quantification of the number of target genes in a community sample. Currently, the standard-curve (SC) method of absolute quantification is widely employed for these kinds of analysis. However,...

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Detalhes bibliográficos
Main Authors: Brankatschk, Robert, Bodenhausen, Natacha, Zeyer, Josef, Bürgmann, Helmut
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3370567/
https://ncbi.nlm.nih.gov/pubmed/22492459
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.07878-11
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