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Randomly broken fragment PCR with 5′ end-directed adaptor for genome walking

Many genome walking methods based on polymerase chain reaction (PCR) are available, including those with and without restriction enzyme modification. Nevertheless, these methods suffer from low reproducibility, inefficiency, and non-specificity. Here, we present a traceable and efficient PCR strateg...

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Bibliografiska uppgifter
Huvudupphovsmän: Xu, Wentao, Shang, Ying, Zhu, Pengyu, Zhai, Zhifang, He, Jing, Huang, Kunlun, Luo, Yunbo
Materialtyp: Artigo
Språk:Inglês
Publicerad: Nature Publishing Group 2013
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC3857568/
https://ncbi.nlm.nih.gov/pubmed/24322619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep03465
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