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Randomly broken fragment PCR with 5′ end-directed adaptor for genome walking
Many genome walking methods based on polymerase chain reaction (PCR) are available, including those with and without restriction enzyme modification. Nevertheless, these methods suffer from low reproducibility, inefficiency, and non-specificity. Here, we present a traceable and efficient PCR strateg...
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| Главные авторы: | , , , , , , |
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| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Nature Publishing Group
2013
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3857568/ https://ncbi.nlm.nih.gov/pubmed/24322619 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep03465 |
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