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Randomly broken fragment PCR with 5′ end-directed adaptor for genome walking

Many genome walking methods based on polymerase chain reaction (PCR) are available, including those with and without restriction enzyme modification. Nevertheless, these methods suffer from low reproducibility, inefficiency, and non-specificity. Here, we present a traceable and efficient PCR strateg...

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Main Authors: Xu, Wentao, Shang, Ying, Zhu, Pengyu, Zhai, Zhifang, He, Jing, Huang, Kunlun, Luo, Yunbo
格式: Artigo
語言:Inglês
出版: Nature Publishing Group 2013
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC3857568/
https://ncbi.nlm.nih.gov/pubmed/24322619
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep03465
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