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SiteFinding-PCR: a simple and efficient PCR method for chromosome walking

In this paper, we present a novel PCR method, termed SiteFinding-PCR, for gene or chromosome walking. The PCR was primed by a SiteFinder at a low temperature, and then the target molecules were amplified exponentially with gene-specific and SiteFinder primers, and screened out by another gene-specif...

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Hlavní autoři: Tan, Guihong, Gao, Yin, Shi, Miao, Zhang, Xinyue, He, Shanping, Chen, Zhangliang, An, Chengcai
Médium: Artigo
Jazyk:Inglês
Vydáno: Oxford University Press 2005
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1182332/
https://ncbi.nlm.nih.gov/pubmed/16077029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gni124
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